Comparative Evaluation of a Novel Phage Protein Ligand Assay and Immunomagnetic Separation Method To Isolate the Seven Top Serogroups of Escherichia coli (O157, O26, O103, O145, O111, O45, and O121) in Foods at Risk

The presence of Shiga toxin-producing Escherichia coli (STEC) in food is a major concern for food safety authorities and industries. Methods for detecting these pathogenic bacteria are crucial. Enrichment of foods for STEC identification has been optimized, but selective concentration of bacteria be...

Full description

Saved in:
Bibliographic Details
Published inJournal of food protection Vol. 80; no. 12; pp. 1973 - 1979
Main Authors Bouvier, M, Miszczycha, S D, Guillarme, X, Tadla, C, Collinet, L, Sergentet, D Thevenot
Format Journal Article
LanguageEnglish
Published Des Moines Elsevier Limited 01.12.2017
Subjects
Agriculture
Antigens
Automation
Bacteria
Comparative studies
Dairy products
E coli
Escherichia coli
Evaluation
Food contamination
Food contamination & poisoning
Food industry
Food safety
Gene expression
Immunomagnetic separation
Infections
Inoculation
Laboratories
Ligands
Meat products
Methods
Pathogens
Phages
Proteins
Separation
Shiga toxin
Thermal cycling
Vegetables
France
Online AccessGet full text

Cover

More Information
Summary:The presence of Shiga toxin-producing Escherichia coli (STEC) in food is a major concern for food safety authorities and industries. Methods for detecting these pathogenic bacteria are crucial. Enrichment of foods for STEC identification has been optimized, but selective concentration of bacteria before isolation still needs to be improved. In the present study, we tested the performance of the VIDAS ESPT detection method against that of the immunomagnetic separation (IMS) method. A preenrichment inoculation was performed to provide a realistic scenario of the contamination that occurs in foods, and the methods were then compared. Results obtained were then confirmed in naturally contaminated foods. Preenrichment inoculation assays revealed that the novel concentration method using phage recombinant proteins or the selective capture of the target top seven STEC serogroups is as specific and sensitive as IMS. Subsequent evaluation of naturally contaminated samples confirmed that the novel concentration method and IMS are equivalent in performance under the conditions tested.
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X..JFP-16-479