Prevalence of [beta]-lactamase genes in domestic washing machines and dishwashers and the impact of laundering processes on antibiotic-resistant bacteria

• Published in: Journal of applied microbiology Vol. 123; no. 6; p. 1396
• Main Authors: Rehberg, L, Frontzek, A, Melhus, Å, Bockmuhl, DP
• Format: Journal Article
• Language: English
• Published: Cambridge Oxford University Press 01.12.2017
• Subjects: Antibiotic resistance; Antibiotics; Bacteria; Bleaches; Coding; Contamination; Cotton; Decontamination; Drug resistance; Genes; Horizontal transfer; Household appliances; Impact resistance; Klebsiella; Methicillin; Outbreaks; Pseudomonas aeruginosa; Reduction; Strains (organisms); Washers & dryers; Washing; Washing machines
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.13574

Aims To investigate the prevalence of [beta]-lactamase genes in domestic washing machines and dishwashers, and the decontamination efficacy of laundering. Methods and Results For the first investigation, swab samples from washing machines (n = 29) and dishwashers (n = 24) were analysed by real-time quantitative PCR to detect genes encoding [beta]-lactamases. To test the impact of laundering on resistant bacteria, cotton test swatches were artificially contaminated with susceptible and resistant strains of Pseudomonas aeruginosa,Klebsiella pneumoniae and Staphylococcus aureus within a second investigation. They were washed in a domestic washing machine with or without activated oxygen bleach (AOB)-containing detergent at 20-50°C. [beta]-Lactamase genes (most commonly of the AmpC- and OXA-type) were detected in 79% of the washing machines and in 96% of the dishwashers and Pseudomonadaceae dominated the microbiota. The level of bacterial reduction after laundering was ≥80% for all Ps. aeruginosa and Kl. pneumoniae strains, while it was only 37-61% for the methicillin-resistant Staph. aureus outbreak strain. In general, the reduction was tendentially higher for susceptible bacteria than for the resistant outbreak strains, especially for Staph. aureus. Conclusions [beta]-Lactamase genes seem to be frequently present in domestic appliances and may pose a potential risk for cross-contamination and horizontal transfer of genes encoding resistance against clinically important [beta]-lactams. In general, higher temperatures and the use of AOB can improve the reduction of antibiotic-resistant bacteria, including Staph. aureus which appears to be less susceptible to the decontamination effect of laundering. Significance and Impact of this Study Data on the presence of antibiotic-resistant bacteria in the domestic environment are limited. This study suggests that [beta]-lactamase genes in washing machines and dishwashers are frequent, and that antibiotic-resistant strains are generally more resistant to the used washing conditions.