Effect of porcine glucagon-like peptides-2 on tight junction in GLP-2Rￂ +ￂ IPEC-J2 cell through the PI3k/Akt/mTOR/p70S6K signalling pathway

Summary Because of rare glucagon-like peptide-2 (GLP-2) receptor (+) cells within the gut mucosa, the molecular mechanisms transducing the diverse actions of GLP-2 remain largely obscure. This research identified the naturally occurring intestinal cell lines that endogenously express GLP-2R and dete...

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Published inJournal of animal physiology and animal nutrition Vol. 101; no. 6; p. 1242
Main Authors Qi, K K, Sun, Y Q, Wan, J, Deng, B, Men, X M, Wu, J, Xu, Z W
Format Journal Article
LanguageEnglish
Published Berlin Wiley Subscription Services, Inc 01.12.2017
Subjects
1-Phosphatidylinositol 3-kinase
AKT protein
Biotechnology
Cell lines
Cytoplasm
Glucagon
Glucagon-like peptide 2
Immunohistochemistry
Intestine
Laminates
Molecular modelling
Mucosa
Peptides
Rapamycin
Signal transduction
Signaling
Tight junctions
TOR protein
Zonula occludens-1 protein
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Summary:Summary Because of rare glucagon-like peptide-2 (GLP-2) receptor (+) cells within the gut mucosa, the molecular mechanisms transducing the diverse actions of GLP-2 remain largely obscure. This research identified the naturally occurring intestinal cell lines that endogenously express GLP-2R and determined the molecular mechanisms of the protective effects of GLP-2-mediated tight junctions (TJ) in GLP-2R (+) cell line. (i) Immunohistochemistry results showed that GLP-2R is localised to the epithelia, laminae propriae and muscle layers of the small and large bowels of newborn piglets. (ii) GLP-2R expression was apparent in the cytoplasm of endocrine cells in IPEC-J2 cell lines. (iii) The protein expressions of ZO-1, claudin-1, occludin, p-PI3K, p-Akt, p-mTOR and p-p70S6K significantly (p < 0.05) increased in GLP-2-treated IPEC-J2 cells, and all of them significantly (p < 0.05) decreased when LY-294002 or rapamycin was added. GLP-2 improves intestinal TJ expression of GLP-2R (+) cells through the PI3k/Akt/mTOR/p70S6K signalling pathway.
ISSN:0931-2439
1439-0396
DOI:10.1111/jpn.12644