SDE3encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis

Post-transcriptional gene silencing (PTGS) provides protection in plants against virus infection and can suppress expression of transgenes. Arabidopsis plants carrying mutations at the SDE3locus are defective in PTGS mediated by a green fluorescent protein transgene. However, PTGS mediated by tobacc...

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Bibliographic Details
Published inThe EMBO journal Vol. 20; no. 8; p. 2069
Main Authors Dalmay, Tamas, Horsefield, Rob, Thomas Hartig Braunstein, Baulcombe, David C
Format Journal Article
LanguageEnglish
Published Heidelberg Blackwell Publishing Ltd 17.04.2001
Subjects
Yeasts
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Summary:Post-transcriptional gene silencing (PTGS) provides protection in plants against virus infection and can suppress expression of transgenes. Arabidopsis plants carrying mutations at the SDE3locus are defective in PTGS mediated by a green fluorescent protein transgene. However, PTGS mediated by tobacco rattle virus (TRV) was not affected by sde3. From these results we conclude that SDE3, like the previously described RNA polymerase encoded by SDE1, acts at a stage in the mechanism that is circumvented when PTGS is mediated by TRV. The product of SDE3is similar to RNA helicase-like proteins including GB110 in mouse and other proteins in Drosophila and humans. These proteins are similar to, but clearly distinct from Upf1p and SMG-2, which are required for nonsense-mediated mRNA decay in yeast and Caenorhabditis elegans and, in the case of SMG-2, for PTGS.
ISSN:0261-4189
1460-2075