Extraction of Herba Lycopi Polysaccharide and Study of Its Antioxidant Activity by Cellulase Method

• Published in: Medicinal plant Vol. 8; no. 3; p. 28
• Main Authors: Huang, Tao, Lu, Wende, Huang, Suoyi, Zhang, Zhaoping, Yan, Zudi, Li, Yunda, Li, Yuanhui, Huang, Ying, Huang, Tingzhang, Yu, Qiaorong, Lin, Mengyao
• Format: Journal Article
• Language: English
• Published: Cranston Wu Chu (USA-China) Science and Culture Media Corporation 01.06.2017
• Subjects: Antioxidants; Cellulase; Chinese medicine; Drug dosages; Edema; Enzymes; Experiments; Extraction processes; Glucose; Innovations; Medical innovations; Phenols; R&D; Research & development; Studies
• ISSN: 2152-3924

2.2 Reagents Herba Lycopi (collected in Youjiang District of Baise City) ; cellulase (activity 40000 U/g) (Tianjin Lihua Enzyme Preparation Co. , Ltd. ); glucose (Guangzhou Aixin Scientific Instrument Co. , Ltd. ) ; DPPH (Sigma Company) ; other reagents were of analytical grade. 3 Methods and results 3.1 Drawing of standard curve 50 mg of standard glucose sample dried (105 °G ) to constant weight was accurately weighed, and the distilled water was used for preparation of 100 ml standard glucose solution. 0, 2, 4, 6, 8, 10 ml of standard glucose solutions were suctioned, respectively, and the volume was set to 50 ml, to get the dilutions of different concentrations. According to the test results, with the extraction rate of polysaccharides as test indicator, the Lg(34) orthogonal experiment was conducted. 3.4 Detection of extraction rate of Herba Lycopi polysaccharides In this study, we used sulfuric acid-phenol method^3' to determine the polysaccharides. 0.5 ml of test solution was placed in 10 ml test tube, and mixed with 1.0 ml of 5% phenol solution and 5.0 ml of concentrated sulfuric acid. The analysis of variance showed that enzyme dosage and enzymolysis temperature had obvious significance in the extraction of Herba Lycopi polysaccharides. [...]the optimum extraction process was A3 B r C2 D3, namely pH of 7, enzyme dosage of 4 ml, 2.5 h of extraction in 40 °C water bath. 3. Received; September 17, 2016 Accepted; April 28, 2017 Supported by Key Traditional Chinese Medicine Discipline Construction Project of State Administration of Traditional Chinese Medicine during the 12th " FiveYear" Plan Period [GuoZhongYiYaoRenJiaoFa(2012)32] ; Guangxi Key Discipline Construction Project [GuiJiaoKeYan(2013)16] ; Guangxi College Science and Technology Innovation Ability Construction Project and Key Laboratory Project of Guangxi's Colleges for the Study of Characteristic Ethnic Medicine in Youjiang River Basin [GuiJiaoKeYan(2014) 14] ; Innovation and Entrepreneurship-Chinese Medicine Teaching Team Project at the Autonomous Regional Level, and Teaching Quality and Teaching Reform Project of Guangxi's Colleges and Universities in 2016 [ GuíJíaoGaoJíao(2015)93&(2016)6] ; College Students' Innovative Training Project of Youjiang Medical University for Nationalities in 2014 ( XCXB201424). * Corresponding author.