C103 OUTDOOR AIR POLLUTION: EPIDEMIOLOGY AND MECHANISMS: Particulate Matter And Ionomycin-Induced Neutrophil Extracellular Traps In Vitro

NETs provide high local concentrations of defense against pathogens, but are also implicated in host tissue injury if not carefully controlled, and are found in elevated levels in the lungs of asthmatic patients. [...]we sought to understand whether particulate matter (PM) derived from diesel combus...

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Bibliographic Details
Published inAmerican journal of respiratory and critical care medicine Vol. 195
Main Authors Wooding, D, Pena, O, Maestre-Batlle, D, Carlsten, C
Format Journal Article
LanguageEnglish
Published New York American Thoracic Society 01.01.2017
Subjects
Air pollution
Neutrophils
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Summary:NETs provide high local concentrations of defense against pathogens, but are also implicated in host tissue injury if not carefully controlled, and are found in elevated levels in the lungs of asthmatic patients. [...]we sought to understand whether particulate matter (PM) derived from diesel combustion alters NET formation in vitro. Samples were stained for flow cytometric analysis against the hallmark components of NETs (DNA stain (DAPI or Hoechst 33342), citrullinated histones (APC-citrullinated anti-histone H3), and myeloperoxidase (FITC-MPO)).
ISSN:1073-449X
1535-4970