A72 MECHANISMS DRIVING FIBROSIS: A Comparison Of Compounds With Claimed Anti-Fibrotic Activity In A Novel Human Fibroblast To Myofibroblast Transition Assay Using Ipf Derived Patient Material

Primary human bronchial fibroblasts, derived from IPF patients (n=7), were isolated and used to establish an FMT assay using TGFß-1 as a stimulus and high content imaging of alpha-smooth muscle actin (aSMA) as a marker for transdifferentiation. To validate this assay, a range of small molecules targ...

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Bibliographic Details
Published inAmerican journal of respiratory and critical care medicine Vol. 195
Main Authors Aarbiou, J, Leeming, D J, Cruwys, S, Gudmann, N S, Brockbank, S, Young, A, De Groot, J
Format Journal Article
LanguageEnglish
Published New York American Thoracic Society 01.01.2017
Subjects
Clinical trials
Fibroblasts
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Summary:Primary human bronchial fibroblasts, derived from IPF patients (n=7), were isolated and used to establish an FMT assay using TGFß-1 as a stimulus and high content imaging of alpha-smooth muscle actin (aSMA) as a marker for transdifferentiation. To validate this assay, a range of small molecules targeting a number of molecular mechanisms, and which displayed a range of efficacies in clinical trials for fibrosis, were tested in concentration response curves in the assays and IC50 values determined.
ISSN:1073-449X
1535-4970