IDENTIFICATION OF NEW ISOLATES (MICRO-ORGANISMS) FROM VINEYARD EPIPHYTIC MICROFLORA USING MALDI-TOF MASS SPECTROMETRY

This paper presents the identification of new isolates (micro-organisms) from mature grapes berries & leaves of specific grape varieties and soil from Oprișor area, using MALDI-TOF mass spectrometry. Red grape varieties taken in study were: Cabernet Sauvignon, two Merlot varieties and Shiraz. In...

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Published inSmart Energy and Sustainable Environment Vol. 19; no. 1; p. 83
Main Authors Bărbulescu, Iuliana Diana, Mihai, Constanța, Begea, Mihaela, Frîncu, Mihai, Grigorică, Liviu, Roceanu, Gabriel, Marinescu, Simona-Ioana, Teodorescu, Răzvan, Tamaian, Radu
Format Journal Article
LanguageEnglish
Published Ramnicu Valcea National Research-Development Institute for Cryogenic and Isotopic Technologies 01.01.2016
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Summary:This paper presents the identification of new isolates (micro-organisms) from mature grapes berries & leaves of specific grape varieties and soil from Oprișor area, using MALDI-TOF mass spectrometry. Red grape varieties taken in study were: Cabernet Sauvignon, two Merlot varieties and Shiraz. In the previous study we identified yeast by MALDI-TOF from this wine area. For each variety of red grapes, leaves and soil samples have been also studied. The main microorganisms from soil were Bacillus sp. from Cabernet area. From Shiraz and Merlot grapes samples the MALDI-TOF score value was 1.26 respectively 1.552, indicating a not reliable identification using MALDI-TOF mass spectrometry. These samples have been also characterized from microscopic and macroscopic point of view. Part of the samples which did not give reliable results by MALDI-TOF mass spectrometry will be the object for the next studies for more precise identification using techniques of molecular taxonomy.
ISSN:2668-957X
2668-957X