Determination of serum aflatoxin B^sub 1^-lysine to evaluate the efficacy of an aflatoxin-adsorbing feed additive in pigs fed an aflatoxin B^sub 1^-contaminated diet

• Published in: Mycotoxin research Vol. 33; no. 2; p. 93
• Main Authors: Di Gregorio, Mayra Carraro, Jager, Alessandra Vincenzi, Souto, Pollyana Cristina; Maggio; Castro, Costa, Aline Alves, Rottinghaus, George Edwin, Passarelli, Danielle, Budiño, Fabio Enrique; Lemos, Corassin, Carlos Humberto, Oliveira, Carlos Augusto; Fernandes
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.05.2017
• Subjects: Adsorbents; Feeds; Hogs
• ISSN: 0178-7888; 1867-1632
• DOI: 10.1007/s12550-016-0267-5

In this study, serum aflatoxin B1 (AFB1)-lysine was determined in order to evaluate the in vivo efficacy of a hydrated sodium calcium aluminosilicate (HSCAS) in pigs fed AFB1. Twenty-four 49-day-old crossbred barrows were maintained in individual cages and allowed ad libitum access to feed and water. A completely randomized design was used with six animals assigned to each of four dietary treatments for 21 days as follows: (A) basal diet (BD), (B) BD supplemented with 0.5 % HSCAS, (C) BD supplemented with 1.1 mg/kg AFB1, and (D) BD supplemented with 0.5 % HSCAS and 1.1 mg/kg AFB1. HSCAS was able to alleviate the toxic effects of AFB1 on pigs and reduce (P < 0.05) the levels of serum AFB1-lysine. Cumulative reductions of adduct yield values, calculated through the equation [(pg AFB1-lysine/mg albumin) / ([mu]g AFB1/kg body weight)], were 53.0, 62.8, and 72.1 after 7, 14, and 21 days of oral exposure, respectively. AFB1-lysine has potential as an AFB1-specific biomarker for diagnostic purposes and for evaluating the efficacy of chemoprotective interventions in pigs.