The Synthesis of 7-Substituted-3-dinitrostyryl Cephalosporins and Their Ability for Detecting Extended Spectrum [beta]-Lactamases (ESBLs)

AbstractWe synthesized 7-substituted-3-(2,4-dinitrostyryl)cephalosporin derivatives which were Nitrocefin analogs, for detecting extended spectrum β-lactamase (ESBL) specifically. HMRZ-86 which has carboxypropyloxyimino group on 7-aminothiazolacetamide substituent were not hydrolyzed by class A, C a...

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Bibliographic Details
Published inJournal of antibiotics Vol. 58; no. 1; p. 69
Main Authors Hanaki, Hideaki, Yamazaki, Hiroaki, Harada, Hidenori, Kubo, Ryoichi, Kobayashi, Takayoshi, Atsuda, Koichiro, Sunakawa, Keisuke
Format Journal Article
LanguageEnglish
Published Tokyo Nature Publishing Group 01.01.2005
Subjects
Acetic acid
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Summary:AbstractWe synthesized 7-substituted-3-(2,4-dinitrostyryl)cephalosporin derivatives which were Nitrocefin analogs, for detecting extended spectrum β-lactamase (ESBL) specifically. HMRZ-86 which has carboxypropyloxyimino group on 7-aminothiazolacetamide substituent were not hydrolyzed by class A, C and D β-lactamases, but it was hydrolyzed by ESBL and metallo β-lactamase (class B), then its color changed from yellow to red. The hydrolysis of metallo β-lactamase was inhibited by adding sodium mercapto acetic acid (SMA). Therefore HMRZ-86 is a useful chromogenic agent to detect ESBL specifically.
ISSN:0021-8820
1881-1469
DOI:10.1038/ja.2005.9