On the effect of [alpha]-tocopherol on the activity of protein kinase C in vitro
We studied the effect of the antioxidant [alpha]-tocopherol on the activity of protein kinase C that was isolated from rat brain, which was used as a model system for investigating a bimodal dose-dependent effect. The enzyme activity was measured photometrically with a luciferase assay for determini...
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Published in | Biophysics (Oxford) Vol. 60; no. 5; p. 859 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Moscow
Springer Nature B.V
01.09.2015
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Subjects | |
Online Access | Get full text |
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Summary: | We studied the effect of the antioxidant [alpha]-tocopherol on the activity of protein kinase C that was isolated from rat brain, which was used as a model system for investigating a bimodal dose-dependent effect. The enzyme activity was measured photometrically with a luciferase assay for determining the amount of ADP that is produced in the phosphorylation reaction. The investigation revealed an inhibitory effect of [alpha]-tocopherol on the activity of protein kinase C in vitro in the concentration range of 10^sup -3^ to 10^sup -6^ M, but the presence of the second peak of the bioeffect with the use of ultra-low doses of [alpha]-tocopherol (10^sup -12^ M or less) was not confirmed, which may be due to the nature of the source of the isolated enzyme. |
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ISSN: | 0006-3509 1555-6654 |
DOI: | 10.1134/S0006350915050115 |