On the effect of [alpha]-tocopherol on the activity of protein kinase C in vitro

We studied the effect of the antioxidant [alpha]-tocopherol on the activity of protein kinase C that was isolated from rat brain, which was used as a model system for investigating a bimodal dose-dependent effect. The enzyme activity was measured photometrically with a luciferase assay for determini...

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Bibliographic Details
Published inBiophysics (Oxford) Vol. 60; no. 5; p. 859
Main Authors Krassova, N E, Ugraitskaya, S V, Penkov, N V, Fesenko, E E
Format Journal Article
LanguageEnglish
Published Moscow Springer Nature B.V 01.09.2015
Subjects
Antioxidants
Biophysics
Enzymes
Kinases
Proteins
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Summary:We studied the effect of the antioxidant [alpha]-tocopherol on the activity of protein kinase C that was isolated from rat brain, which was used as a model system for investigating a bimodal dose-dependent effect. The enzyme activity was measured photometrically with a luciferase assay for determining the amount of ADP that is produced in the phosphorylation reaction. The investigation revealed an inhibitory effect of [alpha]-tocopherol on the activity of protein kinase C in vitro in the concentration range of 10^sup -3^ to 10^sup -6^ M, but the presence of the second peak of the bioeffect with the use of ultra-low doses of [alpha]-tocopherol (10^sup -12^ M or less) was not confirmed, which may be due to the nature of the source of the isolated enzyme.
ISSN:0006-3509
1555-6654
DOI:10.1134/S0006350915050115