Efecto del picloram en la inducción de embriogénesis somática en Pterogyne nitens Tul. "tipa colorada"/[ Picloram effect on the induction of somatic embryogenesis in Pterogyne nitens Tul. "tipa colorada" ]

• Published in: International journal of innovation and applied studies Vol. 11; no. 3; p. 771
• Main Authors: Molina, Maritza Vacca, Avilés, Zulma, Bonomo, María Luisa Cristina, Díaz, Lucía
• Format: Journal Article
• Language: French
• Published: Rabat International Journal of Innovation and Applied Studies 01.06.2015
• ISSN: 2028-9324

Somatic embryogenesis is the process where cells acquire somatic embryogenic competence under specific conditions and interesting tool for clonal propagation. The auxinic herbicide picloram is used as a growth regulator in the induction of callus formation. The "tipa colorada" (Pterogyne nitens) is a legume that belongs to a monospecific genus and is seriously threatened, making it a priority to develop protocols that will produce regeneration of elite material in quantity and quality. This study aimed to evaluate the effect of picloram in the induction of somatic embryogenesis in "tipa colorada", for which the Murashige and Skoog medium was used at 100% of its salt formulation, supplemented with five concentrations of Picloram. At 30 days, color, callus consistency and percent callus formation was evaluated. Character embryogenic callus was determined by microscopic analysis It was possible to induce a 93.78 and 95.22% of embryogenic callus creamy color, with picloram at concentrations of 3.31μM and 4.14μM respectively.