Statistical experimental design to assess the influence of enzymes of nematophagous fungiversushelminths

• Published in: Research in veterinary science Vol. 97; no. 3; p. 527
• Main Authors: Braga, Fabio Ribeiro, de Freitas Soares, Filippe Elias, Araujo, Juliana Milani, da Fonseca, Leandro Abreu, Hiura, Emy, Garschagen Gava, Maylla, Toledo Vieira, Fernanda, da Paz, Jeanne Saraiva, de Carvalho, Lorendane Milena, Faccini, João Victor, de Queiroz, José Humberto, Araújo, Jackson Victor
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Limited 01.12.2014
• Subjects: Chitinase; Design of experiments; Eggs; Enzymes; Fungi; Parasites; Studies; Variables; Veterinary medicine
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2014.09.005

The present work used Plackett-Burman experimental design to assess the influence of enzymes of nematophagous fungiversus Strongyloides westeriand trichostrongylides larvae andPlatynosomum fastosumeggs. The variables studied in the Plackett-Burman design were the proteases and chitinases of AC001 or VC4 as destructive agents ofS. westeriand trichostrongylides larvae, andP. fastosumeggs. All tested enzymes had a significant effect (P< 0.05) on the destruction ofS. westerilarvae. Furthermore, only VC4 and AC001 proteases showed a significant effect (P< 0.05) on the destruction of trichostrongylides larvae. On the other hand, chitinases of VC4 showed the highest significance (P< 0.05) on the destruction ofP. fastosumeggs. It is proposed that statistical planning for the use of enzymes derived from nematophagous fungi is a viable way to elucidate some questions about their mechanism of action.