Homologous prime-boost strategy in neonate mice usingNeisseria lactamica

The aim of the present study was to investigate the immune response to native outer membrane vesicles (NOMVs) ofNeisseria lactamicawith and withoutBordetella pertussis(BP) as adjuvant in intranasal (i.n./i.m) immunization.N. lactamicaNOMVs delivered intranasally (i.n) to BALB/c mice in a final volum...

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Bibliographic Details
Published inVaccine Vol. 27; no. 25-26; p. 3422
Main Authors Ito, André Y, Néri, Simone, Machado, Marta SS, Tunes, Claudia F, De Gaspari, Elizabeth N
Format Journal Article
LanguageEnglish
Published Kidlington Elsevier Limited 26.05.2009
Subjects
Bacteria
Immune response
Immunization
Laboratory animals
Meningitis
Mortality
Proteins
Studies
Vaccines
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Summary:The aim of the present study was to investigate the immune response to native outer membrane vesicles (NOMVs) ofNeisseria lactamicawith and withoutBordetella pertussis(BP) as adjuvant in intranasal (i.n./i.m) immunization.N. lactamicaNOMVs delivered intranasally (i.n) to BALB/c mice in a final volume of 5μl that was gradually introduced with a micropipette, Animals received 1, 2, 3, or 4 doses of antigens at 3, 7, 9 and 12 days after birth. On the 35th day, the animals were immunized intramuscularly (i.m.) with (NOMV) ofN. lactamica. The prime-booster strategy using NOMV ofN. lactamicawith BP as adjuvant in the primer (i.n.) and booster (i.m.) is an effective immunization protocol for inducing humoral immune responses producing IgG antibodies of intermediate to high avidity.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2009.01.114