MITOSIS ANA-THELOPHASE CHROMOSOMAL ABERRATIONS INDUCED BY UV IRRADIATION UNDER THE ANTIOXIDATIVE PROTECTION OF VITAMIN C, BY CALENDULA OFFICINALIS L

Due to the stratospheric ozone layer depletion, the researches focused in the last decades on the study of solar radiations reaching Earth surface. The target of the study was to establish the biological response of Calendula officinalis L. to UV irradiation, under the anti-oxidative protection of v...

Full description

Saved in:
Bibliographic Details
Published inJournal of Experimental and Molecular Biology Vol. 15; no. 2; p. 17
Main Authors Bara, Csilla Iuliana, Campeanu, Mirela Mihaela, Campeanu, Sorin Cristian
Format Journal Article
LanguageEnglish
Published Iasi Universitatea "Alexandru Ioan Cuza" 01.04.2014
Subjects
Antioxidants
Chromosomes
Ultraviolet radiation
Vitamin C
Online AccessGet full text

Cover

More Information
Summary:Due to the stratospheric ozone layer depletion, the researches focused in the last decades on the study of solar radiations reaching Earth surface. The target of the study was to establish the biological response of Calendula officinalis L. to UV irradiation, under the anti-oxidative protection of vitamin C, (which helps next to UV induced anti-oxidative enzymes, in the protection against a large variety of products capable to induce free radicals formation). Our investigations were focused on detecting chromosomal aberrations which occurs during cells in division in meristem root tips, under UV irradiation for 15 or 30 minutes, in the presence or absence of vitamin C, reported to the non- irradiated control samples. Regarding the mitotic index, it could be noticed an inhibition of cell division frequency under UV stress for all irradiated variants, not depending of presence or absence of vitamin C, positively correlated with the increase of irradiation period. Maximal chromosomal aberrations frequency, were induced by UV radiations in the root tips of seedlings germinated in the absence of vitamin C, decreasing in the presence of vitamin C, due to the antioxidant protective role of this. In the absence of UV irradiation, the chromosomal aberrations frequency was lower comparing with irradiated variants, for all seedlings, even if germinated in the presence or absence of vitamin C. Between chromosomal aberrations were detected: bridges, expulsed and retardate chromosomes, fragments.
ISSN:2601-6974
2601-6974