Simultaneous Estimation of Eperisone Hydrochloride and Diclofenac Sodium in Pharmaceutical Dosage Form by RP- HPLC

• Published in: Asian Journal of Research in Chemistry Vol. 7; no. 9; p. 3
• Main Authors: Maniteja, K, Chovatia, Purvil, Naimisha, Jaya, Pavani, Ch, Patil, Prathima
• Format: Journal Article
• Language: English
• Published: Raipur A&V Publications 01.09.2014
• ISSN: 0974-4169; 0974-4150

A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Eperisone Hydrochloride and Diclofenac Sodium in tablet dosage form. The separation was achieved by Nucleosil C18 column with Methanol: Acetonitrile: Triethylamine as mobile phase, at a flow rate of 1.0 ml/min. Detection was carried out at 255 nm. Retention time of Diclofenac Sodium was found to be 2.10 minute and Retention time of Eperisone Hydrochloride was found to be 4.50 minute. The method has been validated for linearity, accuracy, precision and specificity. Linearity for Eperisone Hydrochloride and Diclofenac Sodium were in the range of 30-90μg/ml and 20-60μg/ml respectively. The developed method was validated as per ICH guidelines. Developed method was found to be accurate, precise, specific and rapid for simultaneous estimation of Eperisone Hydrochloride and Diclofenac Sodium in pharmaceutical dosage form. The proposed method was successfully applied for the simultaneous estimation of both the drugs Eperisone Hydrochloride and Diclofenac Sodium in Capsule dosage form.