Induced immune response of DNA vaccine encoding an association MSP1a, MSP1b, and MSP5 antigens ofAnaplasma marginale

The outer membrane proteins ofAnaplasma marginalehave been the focus of research to obtain an improved vaccine against bovine anaplasmosis. We evaluated the capacity of the recombinant plasmids pcDNA-msp1α,pcDNA-msp1β,and pcDNA-mp5 to express MSP1a, MSP1b, and MSP5 proteins, and to determine the imm...

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Published inVaccine Vol. 26; no. 27-28; p. 3522
Main Authors Kano, Flora S, Tamekuni, Katia, Coelho, Adriana L, Garcia, João Luis, Vidotto, Odilon, Itano, Eiko Nakagawa, Vidotto, Marilda C
Format Journal Article
LanguageEnglish
Published Kidlington Elsevier Limited 25.06.2008
Subjects
Cattle
Deoxyribonucleic acid
DNA
Immune response
Immunization
Immunogenicity
Laboratory animals
Membranes
Plasmids
Proteins
R&D
Research & development
Vaccines
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Summary:The outer membrane proteins ofAnaplasma marginalehave been the focus of research to obtain an improved vaccine against bovine anaplasmosis. We evaluated the capacity of the recombinant plasmids pcDNA-msp1α,pcDNA-msp1β,and pcDNA-mp5 to express MSP1a, MSP1b, and MSP5 proteins, and to determine the immunogenicity of BALB/c mice immunized with these plasmids individually or in association. Expression of proteins was confirmed in Vero cells by IFA. The combination of recombinant plasmids showed high antibodies response, produced better induction of Th1 response than individual plasmids, and induced significant proliferation of splenocytes. The mice sera immunized withA. marginaleshowed seroconversion and reacted with all native MSPs, but demonstrated predominance of the humoral IgG1 isotype and did not induce significant proliferation of splenocytes. The use of association of recombinant plasmid can be an effective strategy for the immunoprophylaxis of anaplasmosis.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2008.04.047