Distinct immunoregulatory properties of macrophage migration inhibitory factors encoded byEimeriaparasites and their chicken host

Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that plays an important role in host defense against a variety of microorganisms including protozoan parasites. Interestingly, some microbial pathogens also express a MIF-like protein, although its role in disease pathogenesi...

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Published inVaccine Vol. 29; no. 48; p. 8998
Main Authors Jang, Seung I, Lillehoj, Hyun S, Lee, Sung Hyen, Kim, Duk Kyung, Pagés, Marc, Hong, Yeong Ho, Min, Wongi, Lillehoj, Erik P
Format Journal Article
LanguageEnglish
Published Kidlington Elsevier Limited 08.11.2011
Subjects
Biological properties
Chickens
Chromatography
Cloning
Cytokines
E coli
Gene expression
Infections
Mammals
Membranes
Microorganisms
Migration
Parasites
Proteins
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Summary:Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that plays an important role in host defense against a variety of microorganisms including protozoan parasites. Interestingly, some microbial pathogens also express a MIF-like protein, although its role in disease pathogenesis is not well understood. The aim of this study was to compare anEimeria-encoded MIF (E.MIF) protein with chicken MIF (C.MIF) on the basis of their structural, immunological, and biological properties. E.MIF and C.MIF proteins, each with a glutathione S-transferase epitope tag, were expressed inEscherichia colior COS-7 cells and purified by glutathione affinity chromatography. Rabbit antisera against the purified proteins demonstrated their mutual immunological cross-reactivity on Western blots, and immunolocalized intracellular native E.MIF to theEimeriaschizont, merozoite, and oocyst life cycle stages. HD11 chicken macrophages treatedin vitrowith C.MIF recombinant protein expressed increased levels of transcripts encoding interleukin-6 (IL-6), IL-17, and tumor necrosis factor superfamily member 15 (TNFSF15), but decreased levels of IL-8 transcripts, compared with cells treated with the PBS control; similar treatment with E.MIF only down-regulated IL-8 transcripts. Unlike recombinant E.MIF, C.MIF exhibitedin vitrochemotactic activity for HD11 cells. Conversely, E.MIF, but not C.MIF, enhanced protection against experimentalEimeriainfection, compared with the PBS control. These studies provide evidence for overlapping structural and antigenic properties, but distinct immunoregulatory roles, of E.MIF and C.MIF.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2011.09.038