Investigation of NF- [kappa] B1 and NF- [kappa] BIA Gene Polymorphism in Non-Small Cell Lung Cancer

• Published in: BioMed research international Vol. 2014
• Main Authors: Oltulu, Y M, Coskunpinar, E, Ozkan, G, Aynaci, E, Yildiz, P, Isbir, T, Yaylim, I
• Format: Journal Article
• Language: English
• Published: New York Hindawi Limited 01.01.2014
• Subjects: Cancer therapies; Cell adhesion & migration; Cell culture; Disease; Gene expression; Hospitals; Kinases; Lung cancer; Medical prognosis; Medicine; Patients; Phosphorylation; Proteins; Radiation therapy; Signal transduction; Studies; Thoracic surgery; Transcription factors; Istanbul Turkey
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/530381

Lung cancer is a complex, multifactorial disease which is the leading cause of cancer death in both men and women. NF-κB is a transcription factor which is known to affect the expression of more than 150 genes related to inflammation, lymphocyte activation, cell proliferation, differentiation, and apoptosis, as well as contributing to cell apoptosis and survival. However, NF-κBIA (IκBα) is the inhibitor of the transcription factor. The -94ins/delATTG polymorphism of the NF-κB1 gene promoter region which causes a functional effect and NF-κBIA 3[variant prime]UTR A [arrow right] G polymorphism has been shown to be related to various inflammatory diseases and cancer. Ninety-five NSCLC patients and 99 healthy controls were included in study. The NF-κB1 -94ins/delATTG and NF-κBIA 3[variant prime]UTR A [arrow right] G polymorphism have been studied by using PCR-RFLP method. It was found that the NF-κB1 -94ins/delATTG DD genotype and D allele frequencies were higher in patients than healthy controls and the presence of the DD genotype has a 3.5-fold increased risk of the disease (P: 0.014). This study is the first to investigate the NF-κB1 -94ins/delATTG and NF-κBIA 3[variant prime]UTR A [arrow right] G polymorphism together in the Turkish population. According to the results, the NF-κB1 -94ins/del ATTG promoter polymorphism may have a role in lung carcinogenesis and prognosis.