Study on genetic variations of PPAR[alpha] gene and its effects on thermal tolerance in Chinese Holstein
Peroxisome proliferator-activated receptor alpha (PPAR[alpha]) regulates responses to chemical or physical stress in part by altering expression of genes involved in proteome maintenance. In this research, polymerase chain reaction (PCR) technique was used to amplify 766 and 589 bp fragments of intr...
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Published in | Molecular biology reports Vol. 41; no. 3; p. 1273 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Nature B.V
01.03.2014
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Subjects | |
Online Access | Get full text |
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Summary: | Peroxisome proliferator-activated receptor alpha (PPAR[alpha]) regulates responses to chemical or physical stress in part by altering expression of genes involved in proteome maintenance. In this research, polymerase chain reaction (PCR) technique was used to amplify 766 and 589 bp fragments of intron 3 and 7 of PPAR[alpha] gene in Chinese Holstein (n = 771). Sequencing results showed that three novel single nucleotide polymorphisms (SNPs) were identified at position 44087 (G/A), 65550 (G/A), and 65676(G/A) in the PPAR[alpha] gene. PCR-restriction fragment length polymorphism technology was used to genotype the three SNPs. Association analysis showed that cows with H1H8 (P < 0.05), H2H8 (P < 0.01), H5H7 (P < 0.05), H5H8 (P < 0.05), and H8H8 (P < 0.05) haplotype combinations had lower potassium content in erythrocytes than those with H2H6 haplotype combination. Cows with H1H8, and H8H8 haplotype combinations had lower decrease rate of milk yield than those with H2H6 and H6H8 haplotype combinations (P < 0.05). Cows with H2H8 and H8H8 haplotype combinations had lower rectal temperature than those with H5H8 and H7H7 haplotype combinations (P < 0.05). In conclusion, H8H8 haplotype combination may be advantageous for heat resistance traits in Chinese Holstein cattle.[PUBLICATION ABSTRACT] |
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ISSN: | 0301-4851 1573-4978 |
DOI: | 10.1007/s11033-013-2972-x |