Identification of Canine [alpha]-Lactalbumin

The nucleotide sequence of canine α-lactalbumin cDNA from canine mammary tissue was determined by polymerase chain reaction with degenerate primers.A 742 base pairs nucleotide sequence cloned was similar to the size of mRNA in Northern blot analysis.The cDNA encodes 142 amino acid residues containin...

Full description

Saved in:
Bibliographic Details
Published inJournal of veterinary medical science Vol. 62; no. 11; p. 1217
Main Authors WATANABE, Manabu, TATEYAMA, Susumu, TOGASHI, Takushi, UCHIDA, Kazuyuki, YAMAGUCHI, Ryoji, SHIMIZU, Tsutomu, SUGANO, Sumio
Format Journal Article
LanguageEnglish
Published Tokyo Japan Science and Technology Agency 01.11.2000
Online AccessGet full text

Cover

More Information
Summary:The nucleotide sequence of canine α-lactalbumin cDNA from canine mammary tissue was determined by polymerase chain reaction with degenerate primers.A 742 base pairs nucleotide sequence cloned was similar to the size of mRNA in Northern blot analysis.The cDNA encodes 142 amino acid residues containing the conserved sequence motif of α-lactalbumin, demonstrating the highest homology with pig(73% identity-82% similarity)among the known amino acid sequences of α-lactalbumin.The canine cDNA also showed 71% identity-78% similarity with human, 58-73% with mouse, 60-74% with rat, 67-77% with goat, 66-77% with cattle, and 67-76% with sheep, respectively.
ISSN:0916-7250
1347-7439