Cytotoxicity Induced by Recombinant Human Tumor Necrosis Factor-[alpha] Dependent on the Types of Its Receptors on Canine Cells

• Published in: Journal of veterinary medical science Vol. 60; no. 8; p. 889
• Main Authors: AOKI, Mica, SASAKI, Nobuo, NOMURA, Koichi, KATAMOTO, Hiromu, KUBO, Kihei, KODAMA, Hiroshi, MUKAMOTO, Masafumi, SHIMADA, Terumasa, OHASHI, Fumihito
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Science and Technology Agency 01.08.1998
• ISSN: 0916-7250; 1347-7439

Based on the recent findings that show how recombinant human tumor necrosis factor (rh-TNF)-α has potent antitumor activity on human cancer patients when it locally administrated, we have tested the cytotoxicity of rh-TNF-α on 3 canine cultured cells: (1) canine kidney carcinoma (CKCa-1), (2) mastocytoma and (3) Mardin Darby canine kidney cells (MDCK). The cell surface expression of TNF-α receptors on these canine cells was also determined with anti-human TNF RI and RII polyclonal antibodies. Our data shows that on CKCa-1 which has TNF RI receptors rh-TNF-α induced cytotoxicity. By contrast, it exhibited no toxicity on canine mastocytoma which has mainly RII receptors. The data also suggest actinomycin D (ACT-D), an anticancer antibiotic, enhanced the cytotoxicity of rh-TNF-α. Combined with ACT-D, rh-TNF-α showed the cytotoxicity on MDCK which possessed both TNF RI and RII receptors. The results indicate that the cytotoxicity of rh-TNF-α depends on the presence of TNF RI receptors on canine tumor cells.