Isolation and Characterization of a [delta]5-Desaturase from Oblongichytrium sp

We isolated a cDNA clone with homology to known desaturase genes from Oblongichytrium sp., recently classified as a new genus of thraustochytrids (Labyrinthulomycetes), and found that it encoded δ5-desaturase by its heterologous expression in yeast. The enzyme had higher activity toward 20:4n-3 than...

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Published inBioscience, biotechnology, and biochemistry Vol. 72; no. 8; p. 2224
Main Authors KUMON, Yasuyuki, KAMISAKA, Yasushi, TOMITA, Nao, KIMURA, Kazuyoshi, UEMURA, Hiroshi, YOKOCHI, Toshihiro, YOKOYAMA, Rinka, HONDA, Daiske
Format Journal Article
LanguageEnglish
Published Tokyo Oxford University Press 01.08.2008
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Summary:We isolated a cDNA clone with homology to known desaturase genes from Oblongichytrium sp., recently classified as a new genus of thraustochytrids (Labyrinthulomycetes), and found that it encoded δ5-desaturase by its heterologous expression in yeast. The enzyme had higher activity toward 20:4n-3 than 20:3n-6, indicating that this δ5-desaturase can be used in the production of n-3 polyunsaturated fatty acids in transgenic organisms.
ISSN:0916-8451
1347-6947