Biosynthesis of 2'-O-Methylmyxalamide D in the Myxobacterium Cystobacter fuscus

The biosynthetic gene cluster for the polyene antifungal antibiotic, 2'-O-methylmyxalamide D, was cloned from myxobacterium Cystobacter fuscus AJ-13278. A sequence analysis of the 12.8-kb region in the gene cluster revealed the presence of two type I polyketide synthase genes, mmxB and mmxC. Th...

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Published inBioscience, biotechnology, and biochemistry Vol. 70; no. 3; p. 699
Main Authors FENG, Zhiyang, QI, Jianhua, TSUGE, Takashi, OBA, Yuichi, SAKAGAMI, Youji, OJIKA, Makoto
Format Journal Article
LanguageEnglish
Published Tokyo Oxford University Press 01.03.2006
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Summary:The biosynthetic gene cluster for the polyene antifungal antibiotic, 2'-O-methylmyxalamide D, was cloned from myxobacterium Cystobacter fuscus AJ-13278. A sequence analysis of the 12.8-kb region in the gene cluster revealed the presence of two type I polyketide synthase genes, mmxB and mmxC. The involvement of these two genes in the biosynthesis of 2'-O-methylmyxalamide D was confirmed by a gene disruption experiments. In addition, an S-adenosylmethionine-dependent methyltransferase gene (mmxM) was found downstream of the gene cluster and demonstrated, by a gene disruption analysis, to be responsible for converting the known unmethylated precursor, myxalamide D, into 2'-O-methylmyxalamide D.
ISSN:0916-8451
1347-6947