Insertion of Unmarked DNA Sequences in Multiple Loci of the Bacillus subtilis 168 Genome

• Published in: Bioscience, biotechnology, and biochemistry Vol. 69; no. 5; p. 1036
• Main Authors: UOTSU-TOMITA, Rie, KANEKO, Shinya, TSUGE, Kenji, ITAYA, Mitsuhiro
• Format: Journal Article
• Language: English
• Published: Tokyo Oxford University Press 01.05.2005

The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted...