Insertion of Unmarked DNA Sequences in Multiple Loci of the Bacillus subtilis 168 Genome

The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted...

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Published inBioscience, biotechnology, and biochemistry Vol. 69; no. 5; p. 1036
Main Authors UOTSU-TOMITA, Rie, KANEKO, Shinya, TSUGE, Kenji, ITAYA, Mitsuhiro
Format Journal Article
LanguageEnglish
Published Tokyo Oxford University Press 01.05.2005
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Summary:The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.
ISSN:0916-8451
1347-6947