A Gene Encoding Endo-1, 4-[beta]-glucanase from Bacillus sp. 22-28

• Published in: Bioscience, biotechnology, and biochemistry Vol. 61; no. 2; p. 362
• Main Authors: MIYATAKE, Munetoshi, IMADA, Kiyohisa
• Format: Journal Article
• Language: English
• Published: Tokyo Oxford University Press 01.02.1997

Clones of a gene encoding an endo-1, 4-β-glucanase (EC 3.2.1.4) were obtained from Bacillus sp. 22-28, and the nucleotides were sequenced. A recombinant plasmid, pMK5, included a complete ORF of 2352bp that encoded 783 amino acid residues. Another plasmid, pM3, which showed enzymatic activity in E....