A Gene Encoding Endo-1, 4-[beta]-glucanase from Bacillus sp. 22-28

Clones of a gene encoding an endo-1, 4-β-glucanase (EC 3.2.1.4) were obtained from Bacillus sp. 22-28, and the nucleotides were sequenced. A recombinant plasmid, pMK5, included a complete ORF of 2352bp that encoded 783 amino acid residues. Another plasmid, pM3, which showed enzymatic activity in E....

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Bibliographic Details
Published inBioscience, biotechnology, and biochemistry Vol. 61; no. 2; p. 362
Main Authors MIYATAKE, Munetoshi, IMADA, Kiyohisa
Format Journal Article
LanguageEnglish
Published Tokyo Oxford University Press 01.02.1997
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Summary:Clones of a gene encoding an endo-1, 4-β-glucanase (EC 3.2.1.4) were obtained from Bacillus sp. 22-28, and the nucleotides were sequenced. A recombinant plasmid, pMK5, included a complete ORF of 2352bp that encoded 783 amino acid residues. Another plasmid, pM3, which showed enzymatic activity in E. coli JM109, was also obtained, and it included an incomplete ORF of 1873bp, which lacked the original stop codon and 479 bp of the C-terminal region. The enzymes purified from both of the two types of transformants have shown almost the same properties in comparison with that of the wild type Bacillus sp. 22-28.
ISSN:0916-8451
1347-6947