Formation of Oligosaccharides from Lactose by Bacillus circulans [beta]-Galactosidase

• Published in: Bioscience, biotechnology, and biochemistry Vol. 59; no. 6; p. 1021
• Main Authors: Yanahira, Shuichi, Kobayashi, Tomoko, Suguri, Toshiaki, Nakakoshi, Masamichi, Miura, Susumu, Ishikawa, Hidetoshi, Nakajima, Ichiro
• Format: Journal Article
• Language: English
• Published: Tokyo Oxford University Press 01.06.1995
• ISSN: 0916-8451; 1347-6947

Eleven oligosaccharides formed by a transglycosylation reaction during lactose hydrolysis with Bacillus circulans β-galactosidase were purified by gel permeation chromatography, charcoal chromatography, and HPLC. From the results of methylation analysis, and MS and NMR studies, it was concluded that these oligosaccharides were β-D-Galp-(1->3)-D-Glc, β-D-Galp-(1->6)-D-Glc, β-D-Galp-(1->2)-D-Glc, β-D-Galp-(1->4)-β-D-Galp-(1->4)-D-Glc, β-D-Galp-(1->6)-[β-D-Galp-(1->2)]-D-Glc, β-D-Galp-(1->6)-[β-D-Galp-(1->4)]-D-Glc, β-D-Galp-(1->4)-β-D-Galp-(1->3)-D-Glc, β-D-Galp-(1->4)-β-D-Galp-(1->2)-D-Glc, β-D-Galp-(1->4)-[β-D-Galp-(1->2)]-D-Glc, β-D-Galp-(1->4)-β-D-Galp-(1->6)-D-Glc, β-D-Galp-(1->6)[β-D-Galp-(1->3)]-D-Glc. The last five are newly observed oligosaccharides. The results of a use test (in vitro) by human intestinal bacteria showed that the oligosaccharides containing lactose units were predominantly used by human intestinal bifidobacteria.