The Effects of Dibutyltin(DBT)Dichloride on the Viability and the Productions of Tumor Necrosis Factor [alpha] and Interleukin-12 in Murine Macrophage Cell Line, J774.1

• Published in: Biomedical research on trace elements Vol. 19; no. 1; p. 67
• Main Authors: Tsunoda, Masashi, Yoshida, Tamae, Tsuji, Masayoshi, Zhang, Ying, Sugaya, Chiemi, Inoue, Yoko, Miki, Takeo, Kudo, Yuichiro, Satoh, Toshihiko, Aizawa, Yoshiharu
• Format: Journal Article
• Language: English
• Published: Osaka Japan Science and Technology Agency 01.01.2008
• ISSN: 0916-717X; 1880-1404

Immunotoxicity is one of toxic effects of dibutyltin(DBT)compounds. In our previous study, the inhibitory effects of DBT on the productions of tumor necrosis a(TNFα)and interleukin-1β in a murine macrophage cell line, J774.1, were demonstrated. In this study, the effects of DBT dichloride from 0.125 µM to 2.0 µM on the cell viability and productions of cytokines including TNFα, IL-10 and IL-12p40 were investigated. J774.1 cells were exposed to DBT dichloride at 0, 0.125, 0.25, 0.5, 1.0, 1.5 or 2.0 µM. After 18 hours, lipopolysaccharide was added to each well. Total RNA was extracted from the cells after an additional 6 hours of incubation. Real-time PCR was used to analyze the mRNA expression for TNFα, IL-10, IL-12p40 and glycelaldehyde-3-phosphate dehydrogenase(GAPDH)in J774.1 cells. The cell viabilities were determined and the supernatants were sampled after additional 24 hours of incubation. The concentrations of TNFα, IL-10 and IL-12p40 in the supernatant were determined by ELISA. The mean cell viabilities in the groups exposed to DBT at 0.5 µM and over were significantly lower than that of the control. The mean mRNA expression of IL-12p40 in the 0.25 µM group was significantly higher than that in the control group. The mean concentrations of TNFα in the supernatant in the 1.0, 1.5 and 2.0 µM groups were significantly lower than that of the control, and that in 0.125, 0.25 and 0.5 µM groups were significantly higher. The mean concentration of IL-12p40 in the 0.25 µM group was significantly higher than that of the control and that in the 1.0, 1.5 and 2.0 µM groups were significantly lower. There were no significant differences in mRNA expression and protein level for IL-10. DBT is toxic to macrophages, and the inhibition of the production of cytokines by DBT does not occur for all cytokines.