Lanosterol 14[alpha]-Demethylase (Cytochrome P-45014DM)

Regulation of the activity of lanosterol 14α-demethylase (cytochrome P-45014DM) by dietary cholesterol was studied in rats. In male rats fed a 3% cholesterol diet for 1 and 4 weeks, the activity of 24, 25-dihydrolanosterol 14α-demethylase was decreased in the liver. The cytochrome P-45014DM protein...

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Published inBiological & pharmaceutical bulletin Vol. 18; no. 7; p. 1009
Main Authors SONODA, Yoshiko, AMANO, Chiyuki, ENDO, Masayuki, SATO, Yoshihiro, SEKIGAWA, Yoshio, FUKUHARA, Morio
Format Journal Article
LanguageEnglish
Published Tokyo Japan Science and Technology Agency 01.07.1995
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Summary:Regulation of the activity of lanosterol 14α-demethylase (cytochrome P-45014DM) by dietary cholesterol was studied in rats. In male rats fed a 3% cholesterol diet for 1 and 4 weeks, the activity of 24, 25-dihydrolanosterol 14α-demethylase was decreased in the liver. The cytochrome P-45014DM protein content detected by immunoblotting was also decreased by cholesterol feeding. These results demonstrate that dietary cholesterol acts as a repressive factor for lanosterol 14α-demethylase. Further, the activity was enhanced by preincubation with phosphatase of the enzyme solution from both the control and cholesterol fed rats at the same rate. This result suggests that regulation of the activity involves phosphorylation of this enzyme.
ISSN:0918-6158
1347-5215