Determination of serum cations by ion chromatography

• Published in: Bunseki kagaku Vol. 33; no. 7; p. 347
• Main Authors: SHINTANI, Hideharu, TSUJI, Kusuo, OBA, Takuma
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Science and Technology Agency 01.07.1984
• ISSN: 0525-1931

Many studies on the analysis of serum cations by ion chromatography have been reported. However, as no suitable method for the pretreatment of serum has been found, our purpose has to study the pretreatment of serum for the determination of serum cations. Furthermore, we compared the values determined by ion chromatography with those by atomic absorption method, ion selective electrode method, and colorimetry. For the serum pretreatment, the following methods were compared with one another : (1) Addition of acetonitrile to the serum with the volume ratio of 1 : 1; (2) Addition of 6 M perchloric acid to the serum with the volume ratio of 1: 10; (3) Dilution 10 to 100 fold of the serum with water; (4) Ultrafiltration of serum acidified below pH 3.5. As the result, the method of (4) was found to be the most suitable. The results of the comparison between ion chromatography and other methods on the determined concentrations of serum cations were as follows. The ion selective electrode method was inapplicable because of the interference from the coexisting substances. The values determined by the atomic absorption method seemed to be affected by the volume of serum protein and that of serum lipid, being eight to ten percent by than that by ion selective electrode method. Colorimetry resulted in an almost equal concentration as that by ion chromatography. Ion chromatography was found to be the most suitable for the determination of the free type or the free plus bound type serum cations.