Formation of a reverse parabolic flow profile with electroosmosis in capillary zone electrophoresis and the behavior of zone progress related to the application of a pulsed electric field

The flow profile of electroosmosis in capillary-zone electrophoresis was observed using a microscope-CCD-video system, rectangular capillary (50×1000 μm) and fused-silica capillary (75 μm in diameter). A rhodamine 6 G-methanol solution was used as a fluorescent sample solution. The front zone of ele...

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Bibliographic Details
Published inBunseki kagaku Vol. 46; no. 6; p. 409
Main Authors TSUDA, Takao, KITAGAWA, Shinya, DADOO, Rajeev, N. ZARE, Richard
Format Journal Article
LanguageEnglish
Published Tokyo Japan Science and Technology Agency 01.06.1997
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Summary:The flow profile of electroosmosis in capillary-zone electrophoresis was observed using a microscope-CCD-video system, rectangular capillary (50×1000 μm) and fused-silica capillary (75 μm in diameter). A rhodamine 6 G-methanol solution was used as a fluorescent sample solution. The front zone of electrosomosis forms a reverse parabolic flow profile. The flow speed at the center of a cross-section of capillary was 0.4% lower than that at the near the wall. The progress of frontal zone while an applying pulsed electric field was observed. It was found that the growth and decay is very rapid, less than 1/15 second (which is equal to the time resolution in this experiment).
ISSN:0525-1931