Determination of aluminium in biological samples and drinking water by kinetic-differentiation mode HPLC with fluorescent detection

• Published in: Bunseki kagaku Vol. 49; no. 6; p. 429
• Main Authors: SATO, Makoto, MATSUDA, Jun, MURAYAMA, Harunobu, SHIN, Ryong-Woon, KANEKO, Emiko, YOTSUYANAGI, Takao
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Science and Technology Agency 01.06.2000
• ISSN: 0525-1931

The development of a simple and sensitive method for the determination of aluminium is a subject of growing interest.The objective of this study was to apply kinetic-differentiation mode HPLC with 8-quinolinol to biological and water samples.The aluminium chelate is detected fluorometrically at Ex 370nm and Em 504nm.The most remarkable point of the proposed method is that only aluminium ion respond selectively among many metal ions in the sample matrix.The limit of detection, 1μg/l, is approximately ten-times higher than that of graphitefurnace AAS, which is currently used in routine work.Precautions to prevent aluminium contamination during sample preparation are described.The proposed method has been successfully applied to serum, urine, tap water, and bottled mineral water.The most important practical application is in the treatment of patients on dialysis for whom aluminium accumulation can present serious problems.A comparison of the aluminium concentration between healthy subjects and subjects on dialysis has been difficult because the concentrations in the former group approach the lower limit of those detectable by conventional methods.Using the proposed HPLC, it was determined that the average aluminium concentration in serum was 3μg/l for 240 healthy subjects, and 11μg/l for 196 patients on dialysis.