Highly Sensitive Detection of Bensulfuron-methyl by Time-Resolved Fluoroimmunoassay Using a Tetradentate [beta]-Diketonate Europium Chelate as a Label

A tetradentate β-diketonate europium fluorescent chelate, 4,4'-bis(1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedion-6"-yl)-chlorosulfo-o-terphenyl (BHHCT)-Eu3+, was used as a label for highly sensitive time-resolved fluoroimmunoassay of bensulf...

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Bibliographic Details
Published inAnalytical sciences Vol. 15; no. 2; p. 125
Main Authors YUAN, Jingli, WANG, Guilan, KIMURA, Hiroko, MATSUMOTO, Kazuko
Format Journal Article
LanguageEnglish
Published Tokyo Japan Science and Technology Agency 01.02.1999
Subjects
Europium
Liquid chromatography
Water analysis
Water sampling
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Summary:A tetradentate β-diketonate europium fluorescent chelate, 4,4'-bis(1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedion-6"-yl)-chlorosulfo-o-terphenyl (BHHCT)-Eu3+, was used as a label for highly sensitive time-resolved fluoroimmunoassay of bensulfuron-methyl (BSM). A BSM-BSA (BSA=bovine serum albumin) conjugate was labeled with BHHCT-Eu3+ and the labeled BSM-BSA conjugate was used for competitive immunoassay. The assay was carried out in a 96-well microtiter plate coated with the anti-BSM monoclonal antibody. The method gives the detection limit of 24 pg/ml, which is one to two orders of magnitude lower than those of the conventional HPLC and other methods. The RSD of the present method is less than 10%, and the recovery is in the range of 90 - 110% for BSM water samples.
ISSN:0910-6340
1348-2246