Optical and spectroscopic properties of human whole blood and plasma with and without Y^sub 2^O3 and Nd^sup 3+^:Y^sub 2^O3 nanoparticles

The optical properties of human whole blood and blood plasma with and without Y^sub 2^O3 and Nd^sup 3+^:Y^sub 2^O3 nanoparticles are characterized in the near infrared region at 808 nm using a double integrating sphere technique. Using experimentally measured quantities of diffuse reflectance and di...

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Bibliographic Details
Published inLasers in medical science Vol. 28; no. 6; p. 1559
Main Authors Barrera, Frederick J, Yust, Brian, Mimun, Lawrence C, Nash, Kelly L, Tsin, Andrew T, Sardar, Dhiraj K
Format Journal Article
LanguageEnglish
Published Heidelberg Springer Nature B.V 01.11.2013
Subjects
Blood
Lasers
Nanoparticles
Optical properties
Plasma
Spectrum analysis
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Summary:The optical properties of human whole blood and blood plasma with and without Y^sub 2^O3 and Nd^sup 3+^:Y^sub 2^O3 nanoparticles are characterized in the near infrared region at 808 nm using a double integrating sphere technique. Using experimentally measured quantities of diffuse reflectance and diffuse transmittance, a computational analysis was conducted utilizing the Kubelka-Munk, the Inverse Adding Doubling, and Magic Light Kubelka-Munk and Monte Carlo Methods to determine optical properties of the absorption and scattering coefficients. Room temperature absorption and emission spectra were also acquired of Nd^sup 3+^:Y^sub 2^O3 nanoparticles elucidating their utility as biological markers. The emission spectra of Nd^sup 3+^:Y^sub 2^O3 were taken by exciting the nanoparticles before and after entering the whole blood sample. The emission from the ^sup 4^F^sub 3/2^[arrow right]^sup 4^I^sub 11/2^ manifold transition of Nd^sup 3+^:Y^sub 2^O3 nanoparticles readily propagates through the blood sample at excitation of 808 nm and exhibits a shift in relative intensities of the peaks due to differences in scattering. At 808 nm, in both whole blood and plasma samples, a direct relationship was found with absorption coefficient and Y^sub 2^O3 nanoparticle concentration. Results for the whole blood indicate a small inverse relationship with Y^sub 2^O3 nanoparticle concentration and scattering coefficient and in contrast a direct relation for the plasma.[PUBLICATION ABSTRACT]
ISSN:0268-8921
1435-604X
DOI:10.1007/s10103-013-1268-7