Ang II induce kidney damage by recruiting inflammatory cells and up regulates PPAR gamma and Renin 1 gene: effect of [beta] carotene on chronic renal damage

• Published in: Journal of thrombosis and thrombolysis Vol. 36; no. 3; p. 277
• Main Authors: Kaliappan, Gopal, Nagarajan, P, Moorthy, Ramya, Kalai Gana Selvi, S, Avinash Raj, T, Mahesh Kumar, Jerald
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.10.2013
• ISSN: 0929-5305; 1573-742X
• DOI: 10.1007/s11239-012-0835-2

Antioxidants are widely used for prevention of diseases associated with oxidative stress and ischemic disorder. We investigated the hypothesis of antioxidants ([alpha]-tocopherol and [beta]-carotene) can suppress the renal disorder in apo E-/-mice. Renal damage induced by chronic infusion of Angiotensin II (Ang II) into 4 month old male apo E-/-mice. After that the mice were treated with diet enriched [alpha] tocopherol and [beta] carotene (800 mg/kg) for 150 days. Ang II treated kidney showed polycystic appearance with accumulation of clear fluid and constriction of renal artery and renal vein was noticed. Vacuolar/cystic degeneration as well as inflammatory reactions was noticed in the tubules/glomerulus of Ang II treated mice. [beta] carotene treated mice showed enormous numbers of regenerated tubules in the kidney and over expression of ICAM proteins in the regenerated tubules. CD 45.2, MAC 3 proteins were over expressed in the inflammatory cells infiltrated into the tubular region of Ang II treated kidney. Gene expression studies revealed up regulation of Renin 1 (Ren 1) and PPARγ genes in the kidney of Ang II treated animals, but the [beta] carotene treatment controlled the expression of these genes in the regenerated kidneys. [beta] carotene may have protective effective on chronic renal disorder. It may repress the inflammatory genes (Ren 1, PPARγ) to achieve the protective effect on Ang II induced renal damage.[PUBLICATION ABSTRACT]