Influence of CdCl^sub 2^ and CdSO^sub 4^ supplementation on Cd distribution and ligand environment in leaves of the Cd hyperaccumulator Noccaea (Thlaspi) praecox

The aim was to investigate whether different Cd salts in the nutrient solution of the Cd/Zn hyperaccumulator Noccaea (Thlaspi) praecox alter leaf Cd distribution and Cd ligand environment, and plant fitness. Plants were grown for 8 weeks with 100/300 [mu]M CdCl^sub 2^ or CdSO^sub 4^. Leaf biomass, a...

Full description

Saved in:
Bibliographic Details
Published inPlant and soil Vol. 370; no. 1-2; p. 125
Main Authors Koren, Spela, Arcon, Iztok, Kump, Peter, Necemer, Marijan, Vogel-mikus, Katarina
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.09.2013
Subjects
Absorption
Cadmium
Leaves
Ligands
Nutrient concentrations
Plant biology
Plant growth
Salinity
X-ray fluorescence
Online AccessGet full text

Cover

More Information
Summary:The aim was to investigate whether different Cd salts in the nutrient solution of the Cd/Zn hyperaccumulator Noccaea (Thlaspi) praecox alter leaf Cd distribution and Cd ligand environment, and plant fitness. Plants were grown for 8 weeks with 100/300 [mu]M CdCl^sub 2^ or CdSO^sub 4^. Leaf biomass, and total chlorophyll, anthocyanin, Cd, Cl, S and P concentrations were monitored. Cd localisation and ligand environment in leaves were analysed using quantitative synchrotron-based micro-X-ray fluorescence imaging, and Cd K-edge X-ray absorption fine structure and Cd L^sub 3^-edge micro-X-ray absorption near-edge structure measurements. Cd uptake and plant fitness were comparable for CdCl^sub 2^ and CdSO^sub 4^ treatments, and depended on applied Cd concentration. In all treatments, Cd preferentially accumulated with high concentrations of Cl in vacuoles of large vacuolarised epidermal cells, bound mainly to oxygen-based (O)-ligands. In the mesophyll of CdCl^sub 2^- treated plants, Cd was preferentially sequestered in vacuoles, while for CdSO^sub 4^, Cd accumulated preferentially in the apoplast. In the symplast, O-ligands increased with increasing Cd concentrations; in the apoplast, sulphur-based (S)-ligands prevailed. Cd partitioning between leaf mesophyll apoplast and symplast and the Cd ligand environment in N. praecox depend on the Cd salt type and concentration added to the nutrient solution.[PUBLICATION ABSTRACT]
ISSN:0032-079X
1573-5036
DOI:10.1007/s11104-013-1617-0