PCR-Based Detection of Extended-Spectrum [beta]-Lactamases (bla^sub CTX-M-1^ and bla^sub TEM^) in Escherichia coli, Salmonella spp. and Klebsiella pneumoniae Isolated from Pigs in North Eastern India (Mizoram)

Cephalosporins are major antimicrobials used to treat serious infections. However, their effectiveness is being compromised by the emergence of extended-spectrum [beta]-lactamases (ESBLs). A total of 138 enteric bacteria were isolated from 53 faecal samples of pigs collected from different districts...

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Published inIndian journal of microbiology Vol. 53; no. 3; p. 291
Main Authors Lalzampuia, H, Dutta, T K, Warjri, Iadarilin, Chandra, Rajesh
Format Journal Article
LanguageEnglish
Published New Delhi Springer Nature B.V 01.09.2013
Subjects
Animals
Annealing
Antibiotics
Antimicrobial agents
Bacteria
Curing
E coli
Genes
Hogs
Plasmids
Salmonella
India
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Summary:Cephalosporins are major antimicrobials used to treat serious infections. However, their effectiveness is being compromised by the emergence of extended-spectrum [beta]-lactamases (ESBLs). A total of 138 enteric bacteria were isolated from 53 faecal samples of pigs collected from different districts of Mizoram, of which 102 (73.91 %) were Escherichia coli, 26 (18.84 %) were Salmonella spp. and 10 (7.25 %) were Klebsiella pneumoniae. Phenotypic confirmatory test (Double Discs Synergy Test) showed that 8 (5.80 %) E. coli isolates were ESBLs producer. PCR analysis confirmed that out of the eight isolate, 7 (5.07 %) harboured bla ^sub CTX-M-1^ gene and/or bla ^sub TEM^ gene. Of the eight positive isolates, 7 (5.07 %) and 3 (2.17 %) were found to be positive for bla ^sub CTX-M-1^ gene and bla ^sub TEM^ gene, respectively, of which 3 (2.17 %) isolates were positive for both the genes. Only 4 (2.90 %) E. coli isolates carried bla ^sub CTX-M-1^ gene alone. Agarose gel electrophoresis showed that all the isolates were carrying plasmids ranging between 0.9 and ~30 kb. Out of the seven isolates positive for bla ^sub CTX-M-1^ and/or bla ^sub TEM^, 2 (1.84 %) isolates were confirmed for bla ^sub CTX-M-1^ gene in their plasmid. Only one E. coli isolate was found to be positive for both the genes in its plasmid. The resistance plasmid could not be transferred to a recipient by in vitro horizontal gene transfer method.[PUBLICATION ABSTRACT]
ISSN:0046-8991
0973-7715
DOI:10.1007/s12088-013-0378-z