RP - HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ANALYSIS OF CLOBAZAM IN PHARMACEUTICAL DOSAGE FORMS

A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of clobazamin tablet dosage form. Isocratic elution at a flow rate of 1ml min-1 was employed on a symmetry C18 column at ambient temperature. The mobile phase consisted of Methanol:water:OPA(0.1%) 35:40:25 (v/v...

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Published inJournal of atoms and molecules Vol. 1; no. 1; p. 55
Main Authors Kumari, K Gowri Bala, Vanilatha, S, Theresa, M Mary, Prasanna, N, Kumari, D Shantha, Harika, B, Sirisha, P, Xavier, M Archana
Format Journal Article
LanguageEnglish
Published Chennai Journal of Atoms and Molecules 01.11.2011
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Summary:A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of clobazamin tablet dosage form. Isocratic elution at a flow rate of 1ml min-1 was employed on a symmetry C18 column at ambient temperature. The mobile phase consisted of Methanol:water:OPA(0.1%) 35:40:25 (v/v/v). The UV detection wavelength was at 239nm.Linearity was observed in concentration range of 4-16ppm. The retention time for Clobazam was 4.71 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Clobazam in pharmaceutical dosage forms. [PUBLICATION ABSTRACT]
ISSN:2277-1247