Siderophore-Mediated Iron Uptake Promotes Yeastâ[euro]"Bacterial Symbiosis

In the present study, siderophore produced by the marine yeast Aureobasidium pullulans was characterized as hydroxamate by chemical and bioassays. The hydroxamate assignment was supported by the appearance of peaks at 1,647.21â[euro]"1,625.99Â cm^sup â '1^ and at 1,435.04Â cm^sup â '1...

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Bibliographic Details
Published inApplied biochemistry and biotechnology Vol. 168; no. 8; p. 2170
Main Authors Murugappan, Rm, Karthikeyan, M, Aravinth, A, Alamelu, Mr
Format Journal Article
LanguageEnglish
Published Totowa Springer Nature B.V 01.12.2012
Subjects
Bacteria
Bioassays
Iron
Symbiosis
Yeast
Yeasts
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Summary:In the present study, siderophore produced by the marine yeast Aureobasidium pullulans was characterized as hydroxamate by chemical and bioassays. The hydroxamate assignment was supported by the appearance of peaks at 1,647.21â[euro]"1,625.99Â cm^sup â '1^ and at 1,435.04Â cm^sup â '1^ in the infrared spectrum. The purified siderophore exhibited specific growth-promoting activity under iron-limited conditions for siderophore auxotrophic probiotic bacteria. Cross-utilization of siderophore indicates a symbiotic relationship between the yeast A. pullulans and the selected probiotic bacterial strains. Statistical optimization of medium components for improved siderophore production in A. pullulans was depicted by response surface methodology. The shift in UVâ[euro]"Vis spectroscopy indicates the photoreactive property and subsequent oxidative cleavage of purified siderophore on exposure to sunlight.[PUBLICATION ABSTRACT]
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-012-9926-y