Fluorescence Quenching by Intercalation of a Pyrene Group Tethered to an N^sup 4^-modified Cytosine in Duplex DNA

A series of duplex DNA oligomers was prepared that contain a pyrene chromophore linked by a trimethylene chain (-(CH...)...) to N... of a cytosine. The pyrene group stabilizes the DNA as evidenced by an increase in melting temperature. The absorption spectrum of the linked pyrene chromophore shows a...

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Bibliographic Details
Published inPhotochemistry and photobiology Vol. 89; no. 2; p. 332
Main Authors Avirah, Rekha R, Schuster, Gary B
Format Journal Article
LanguageEnglish
Published Lawrence Blackwell Publishing Ltd 01.03.2013
Subjects
Deoxyribonucleic acid
DNA
Melting
Photochemistry
Polycyclic aromatic hydrocarbons
Temperature effects
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Summary:A series of duplex DNA oligomers was prepared that contain a pyrene chromophore linked by a trimethylene chain (-(CH...)...) to N... of a cytosine. The pyrene group stabilizes the DNA as evidenced by an increase in melting temperature. The absorption spectrum of the linked pyrene chromophore shows a temperature-dependent shift and there is also a strong induced circular dichroism spectrum attributed to the pyrene group. The fluorescence of the pyrene chromophore is strongly quenched at room temperature by linkage to the DNA, but it increases above the melting temperature. We attribute these observations to intramolecular intercalation of the pyrene group at a base pair adjacent to its linkage site at cytosine. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:0031-8655
1751-1097