Detection of amplified SRY gene by a novel electrochemical biosensor based on gold nanoparticles
This paper describes an electrochemical hybridization biosensor that investigates DNA hybridization on the modified Glassy Carbon Electrode (GCE). GCE was modified by a mixture of Au nanoparticles (AuNPs) and calf thymus DNA (ctDNA). SH-ssDNA was self-assembled on a modified electrode, which is a se...
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Published in | Scientia Iranica. Transaction F, Nanotechnology Vol. 19; no. 3; p. 913 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Tehran
Sharif University of Technology
01.06.2012
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Subjects | |
Online Access | Get full text |
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Summary: | This paper describes an electrochemical hybridization biosensor that investigates DNA hybridization on the modified Glassy Carbon Electrode (GCE). GCE was modified by a mixture of Au nanoparticles (AuNPs) and calf thymus DNA (ctDNA). SH-ssDNA was self-assembled on a modified electrode, which is a segment of the sex determining region of the Y (SRY) gene. This electrode can detect the complementary and non-complementary strand of SRY gene, by Methylene Blue (MB), as an electroactive label, and the Differential Pulse Voltammetry (DPV) technique. The modified GCE for the complementary DNA had a linear dynamic range of 0.5-30.0 ppm. This is the first report of sex determination by the electrochemical biosensor of a Polymerase Chain Reaction (PCR) product. Also, this biosensor showed stability on thermal denaturation for five sequential denaturation processes. [PUBLICATION ABSTRACT] |
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