Identification and regional localization of a human IMP dehydrogenase-like locus (IMPHDL1) at 16p13. 13

• Published in: Genomics (San Diego, Calif.) Vol. 18:3
• Main Authors: Doggett, N.A., Tesmer, J.G., Duesing, L.A., Callen, D.F., Chen, Z.L., Moore, S., Stallings, R.L.
• Format: Journal Article
• Language: English
• Published: United States 01.12.1993
• Subjects: 550400 - Genetics; BASIC BIOLOGICAL SCIENCES; CHROMOSOMES; ENZYMES; GENES; GENETIC MAPPING; HUMAN CHROMOSOME 16; HUMAN CHROMOSOMES; MAPPING; NUCLEOTIDE DEHYDROGENASES; ORGANIC COMPOUNDS; OXIDOREDUCTASES; PROTEINS
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/S0888-7543(05)80374-X

Sequence-tagged sites (STS)s are versatile chromosomal markers for a variety of genome mapping efforts. In this report, the authors describe a randomly generated STS (323F4) from human chromosome 16 genomic DNA that has 90.0% sequence identity to the type I human inosine-5[prime]-monophosphate dehydrogenase (IMPDH1) gene and 72% identity to the type II human inosine-5[prime]-monophosphate dehydrogenase (IMPDH2) gene. Additional sequencing by primer walking has provided a total of 1380 bp of the human chromosome 16 sequence. The IMPDH-like sequence 323F4 was regionally localized by PCR analysis of a panel of somatic cell hybrids containing different portions of human chromosome 16 to 16p13.3-13.12, between the breakpoints found in hybrids CY196/CY197 and CY198. This regional mapping assignment was further refined to subband 16p13.3 by high-resolution fluorescence in situ hybridization using cosmid 323F4 as a probe. The authors conclude that a third, previously undescribed IMPDH locus, termed IMPDHL1, exists at human chromosome 16p13.13. 11 refs., 2 figs.