The L-isoaspartyl/D-aspartyl protein methyltransferase gene (PCMT1) maps to human chromosome 6q22. 3-6. q24 and the syntenic region of mouse chromosome 10

The authors have mapped the genes for the human and mouse L-isoaspartyl./D-aspartyl protein carboxyl methyltransferase (EC 2.1.1.77) using cDNA probes. They determined that the human gene is present in chromosome 6 by Southern blot analysis of DNA from a panel of mouse-human somatic cell hybrids. In...

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Published inGenomics (San Diego, Calif.) Vol. 14:4
Main Authors MacLaren, D.C., O'Conner, C.M., Xia, Yu-Rong, Mehrabian, M., Klisak, I., Sparkes, R.S., Lusis, A.J., Clarke, S.
Format Journal Article
LanguageEnglish
Published United States 01.12.1992
Subjects
550400 - Genetics
AMINO ACIDS
ASPARTIC ACID
BASIC BIOLOGICAL SCIENCES
BIOTECHNOLOGY
CARBON-GROUP TRANSFERASES
CARBOXYLIC ACIDS
CHROMOSOMES
DNA HYBRIDIZATION
ENZYMES
GENES
GENETIC ENGINEERING
GENETIC MAPPING
HUMAN CHROMOSOME 6
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
METHYL TRANSFERASES
NUCLEIC ACID HYBRIDIZATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RFLPS
TRANSFERASES
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Summary:The authors have mapped the genes for the human and mouse L-isoaspartyl./D-aspartyl protein carboxyl methyltransferase (EC 2.1.1.77) using cDNA probes. They determined that the human gene is present in chromosome 6 by Southern blot analysis of DNA from a panel of mouse-human somatic cell hybrids. In situ hybridization studies allowed confirmation of this identification and further localized the human gene (PCMT1) to the 6q22.3-6q24 region. By analyzing the presence of an EcoRI polymorphism in DNA from backcrosses of C57BL/6J and Mus spretus strains of mice, they localized the mouse gene (Pcmt-1) to chromosome 10, at a position 8.2 [+-] 3.5 cM proximal to the Myb locus. This region of the mouse chromosome is homologous to the human 6q24 region. 32 refs., 2 figs., 2 tabs.
ISSN:0888-7543
1089-8646
DOI:10.1016/S0888-7543(05)80104-1