sup 14 C-2-deoxyglucose uptake in the ground squirrel brain during entrance to and arousal from hibernation

Neuronal activity underlying various phases of the mammalian hibernation cycle was investigated using the {sup 14}C-2-deoxyglucose (2DG) method. Relative 2DG uptake (R2DGU) values were computed for 96 brain regions across 7 phases of the hibernation cycle: euthermia, 3 body temperature (Tb) interval...

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Bibliographic Details
Published inThe Journal of neuroscience Vol. 10:7
Main Authors Kilduff, T.S., Miller, J.D., Radeke, C.M., Sharp, F.R., Heller, H.C.
Format Journal Article
LanguageEnglish
Published United States 01.07.1990
Subjects
550501 - Metabolism- Tracer Techniques
ALDEHYDES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BASIC BIOLOGICAL SCIENCES
BODY
BRAIN
CARBOHYDRATES
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
GLUCOSE
HEXOSES
HIBERNATION
LABELLED COMPOUNDS
MAMMALS
MATHEMATICS
MEMBRANE TRANSPORT
METABOLISM
MONOSACCHARIDES
MULTIVARIATE ANALYSIS
NERVE CELLS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
RODENTS
SACCHARIDES
SOMATIC CELLS
SQUIRRELS
STATISTICS
VERTEBRATES
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Summary:Neuronal activity underlying various phases of the mammalian hibernation cycle was investigated using the {sup 14}C-2-deoxyglucose (2DG) method. Relative 2DG uptake (R2DGU) values were computed for 96 brain regions across 7 phases of the hibernation cycle: euthermia, 3 body temperature (Tb) intervals during entrance into hibernation, stable deep hibernation, and 2 Tb intervals during arousal from hibernation. Multivariate statistical techniques were employed to identify objectively groups of brain regions whose R2DGU values showed a similar pattern across all phases of hibernation. Factor analysis revealed that most of the variability in R2DGU values for the 96 brain regions across the entire cycle could be accounted for by 3 principal factors. These factors could accurately discriminate the various phases of hibernation on the basis of the R2DGU values alone. Three hypothalamic and 3 cortical regions were identified as possibly mediating the entrance into hibernation because they underwent a change in R2DGU early in entrance into hibernation and loaded strongly on one of the principal factors. Another 4 hypothalamic regions were similarly identified as possibly causally involved in the arousal from hibernation. These results, coupled with characteristic changes in ordinal rank of the 96 brain regions in each phase of hibernation, support the concept that mammalian hibernation is an active, integrated orchestration of neurophysiological events rather than a state entered through a passive process.
ISSN:0270-6474
1529-2401