Immunoglobulin. gamma. light-chain-related genes 14. 1 and 16. 1 are expressed in pre-B cells and may encode the human immunoglobulin. omega. light-chain protein

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 86:14
• Main Authors: Hollis, G.F., Evans, R.J., Stafford-Hollis, J.M., Korsmeyer, S.J., McKearn, J.P.
• Format: Journal Article
• Language: English
• Published: United States 01.07.1989
• Subjects: 550201 - Biochemistry- Tracer Techniques; AMINO ACIDS; ANIMAL CELLS; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DNA; DNA SEQUENCING; DRUGS; EVEN-ODD NUCLEI; GENE REGULATION; GENES; GLOBULINS; IMMUNOGLOBULINS; ISOTOPES; LEUKOCYTES; LIGHT NUCLEI; LIPOTROPIC FACTORS; LYMPHOCYTES; MATERIALS; METHIONINE; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PHOSPHORUS 32; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; RECOMBINANT DNA; SOMATIC CELLS; STRUCTURAL CHEMICAL ANALYSIS; SULFUR 35; SULFUR ISOTOPES; TRANSCRIPTION; TUMOR CELLS
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.86.14.5552

Human pre-B cells, which produce immunoglobulin heavy chain but do not produce immunoglobulin light chain, are shown to contain a 1-kilobase transcript homologous to immunoglobulin {lambda} light-chain genes. Detailed analysis of RNA and cDNA clones derived from these transcripts reveals that they originate from the distinct immunoglobulin {lambda}-like genes 14.1/16.1. Sequence analysis of these clones reveals a long open reading frame, beginning with an ATG, capable of encoding a protein of 214 amino acids with an unprocessed molecular weight of 22,944. The C-terminal half of this predicted protein is highly homologous to immunoglobulin {lambda} light-chain joining and constant region protein sequence, while the amino-terminal end does not share homology with variable regions. Antisera raised against a peptide whose sequence was predicted from the 14.1 cDNA sequence identifies a 22-kDa protein in human pre-B cells. Immunoprecipitation of immunoglobulin {mu}-chain from these pre-B cells with anti-immunoglobulin {mu} antibody coprecipitates a 22-kDa protein, which is a candidate for the human immunoglobulin {omega} light-chain protein and may be the protein product of the 14.1/16.1 genes.