Engineering a Non–Natural Photoenzyme for Improved Photon Efficiency

We developed a novel HTS engineering platform to optimize photoenzymatic activity. The improvements in variants were correlated to an increase in enzymatic photon efficiency. Here, transient absorption spectroscopy revealed a shift from a stepwise to a concerted mechanism. The platform was expanded...

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Bibliographic Details
Published inAngewandte Chemie (International ed.) Vol. 61; no. 2
Main Authors Nicholls, Bryce T., Oblinsky, Daniel G., Kurtoic, Sarah I., Grosheva, Daria, Ye, Yuxuan, Scholes, Gregory D., Hyster, Todd K.
Format Journal Article
LanguageEnglish
Published United States Wiley 05.11.2021
Subjects
BASIC BIOLOGICAL SCIENCES
biocatalysis
directed evolution
ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
photocatalysis
photophysics
transient absorption
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Summary:We developed a novel HTS engineering platform to optimize photoenzymatic activity. The improvements in variants were correlated to an increase in enzymatic photon efficiency. Here, transient absorption spectroscopy revealed a shift from a stepwise to a concerted mechanism. The platform was expanded to improve the synthesis of γ, δ, ϵ-lactams, and acyclic amides.
Bibliography:USDOE Office of Science (SC), Basic Energy Sciences (BES)
SC0019370
ISSN:1433-7851
1521-3773