Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis

Synechococcus elongatus UTEX 2973 is the fastest growing cyanobacterium characterized to date. Its genome was found to be 99.8% identical to S. elongatus 7942 yet it grows twice as fast. Current genome-to-phenome mapping is still poorly performed for non-model organisms. Even for species with identi...

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Bibliographic Details
Published inBiotechnology for biofuels Vol. 10; no. 1
Main Authors Abernathy, Mary H., Yu, Jingjie, Ma, Fangfang, Liberton, Michelle, Ungerer, Justin, Hollinshead, Whitney D., Gopalakrishnan, Saratram, He, Lian, Maranas, Costas D., Pakrasi, Himadri B., Allen, Doug K., Tang, Yinjie J.
Format Journal Article
LanguageEnglish
Published Netherlands Springer Science + Business Media 16.11.2017
Subjects
13C labeling experiments
BASIC BIOLOGICAL SCIENCES
Channeling
Energy charge
Glycogen
Metabolites
Photobioreactor
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Summary:Synechococcus elongatus UTEX 2973 is the fastest growing cyanobacterium characterized to date. Its genome was found to be 99.8% identical to S. elongatus 7942 yet it grows twice as fast. Current genome-to-phenome mapping is still poorly performed for non-model organisms. Even for species with identical genomes, cell phenotypes can be strikingly different. To understand Synechococcus 2973’s fast-growth phenotype and its metabolic features advantageous to photo-biorefineries, 13C isotopically nonstationary metabolic flux analysis (INST-MFA), biomass compositional analysis, gene knockouts, and metabolite profiling were performed on both strains under various growth conditions.
Bibliography:USDOE Office of Science (SC), Biological and Environmental Research (BER)
DESC0012722; AE-AR0000202; AR0000202; SC0012722
ISSN:1754-6834
1754-6834
DOI:10.1186/s13068-017-0958-y