Description of Rhodanobacter denitrificans sp. nov., isolated from nitrate-rich zones of a contaminated aquifer

Bacterial strains 2APBS1T and 116-2 were isolated from the subsurface of a nuclear legacy waste site where sediments are co-contaminated with large amounts of acidity, nitrate, metal radionuclides and other heavy metals. A combination of physiological and genetic assays indicated that these strains...

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Bibliographic Details
Published inInternational journal of systematic and evolutionary microbiology
Main Authors Prakash, Om, Green, Stefan, Jasrotia, Puja, Overholt, Will, Canion, Andy, Watson, David B, Brooks, Scott C, Kostka, Joel
Format Journal Article
LanguageEnglish
Published United States 01.01.2011
Subjects
AQUIFERS
BASIC BIOLOGICAL SCIENCES
CARBOXYLIC ACIDS
DENITRIFICATION
ENVIRONMENTAL SCIENCES
GENES
GENETICS
HEAVY METALS
HYBRIDIZATION
PH VALUE
RADIOISOTOPES
SEDIMENTS
STRAINS
UBIQUINONE
WASTES
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Summary:Bacterial strains 2APBS1T and 116-2 were isolated from the subsurface of a nuclear legacy waste site where sediments are co-contaminated with large amounts of acidity, nitrate, metal radionuclides and other heavy metals. A combination of physiological and genetic assays indicated that these strains represent the first members of the Rhodanobacter genus shown to be capable of complete denitrification. Cells of strain 2APBS1T and 116-2 were Gram negative, non-spore-forming, rods, 3-5 micro;m long and 0.25-0.5 m in diameter. The isolates were facultative anaerobes, and had temperature and pH optima for growth at 30 C and pH 6.5, respectively, and could tolerate up to 2.0 % NaCl, though growth improved in its absence. Strains 2APBS1T and 116-2 contained fatty acid profiles and 100 % Q-8 ubiquinone, that are characteristic features of the genus Rhodanobacter. Although strains 2APBS1T and 116-2 share high SSU rRNA gene sequence similarity to R. thiooxydans (>99%), DNA-DNA hybridization values were substantially below the 70% threshold used to designate novel species. Thus, based on genotypic, phylogenetic, chemotaxonomic and physiological differences, strains 2APBS1T and 116-2 are considered to represent a novel species of the genus Rhodanobacter, for which the name Rhodanobacter denitrificans sp. nov is proposed. The type strain is 2APBS1T (=DSM 23569T =JCM 17641T). Strain 116-2 (=DSM 24678 = JCM 17642) is a reference strain.
Bibliography:DE-AC05-00OR22725
USDOE Office of Science (SC)
ISSN:1466-5026
1466-5034